Mechanism(s) of long-term humoral immunity and immune suppression induced by wildtype Measles virus in rhesus macaques

Abstract Measles virus (MeV) causes a highly contagious systemic viral infection that remains an important cause of morbidity and mortality in children worldwide. While recovery from measles induces life-long protective immunity, it also immune amnesia which can lead to higher susceptibility other infections. To better understand the long-term responses induced by MeV vaccine versus wildtype (WT) MeV, first, we studied rhesus macaques for six months after WT or Measles-Mumps-Rubella (MMR) inoculation. We quantified total antibody secreting cells (ASC) MeV-specific ASCs peripheral blood mononuclear (PBMCs) using well-characterized house methods ELISpot; assessed IgG, avidity, plaque reduction neutralization titers. Second, whether loss memory post is linked eviction B bone marrow (BM) into circulation. BM PBMCs live attenuated (LAMV) vaccinated monkeys were cultured supernatant isotype antigen specificity ELISA. PBMC cultures produced detectable amount recognition profiles will be compared via Virscan, phage-display technology epitope mapping. A novel panel non-human primate (NHP) pathogen peptides representing Herpesviruses, Retroviruses, Adenoviruses, Pneumoviruses, Enteroviruses developed expressed system with plans use them characterize changes production. hypothesis robust response accompanied long-lived plasma niches resulting Ab diversity does not occur LAMV. Supported grants NIH (5R01AI153140-03)